fig , confocal fluorescence images of bt cells stained with mitotracker red after exposure for lohrs to dna green complexed with cdqasomes left column circular mlspdna conjugate, right column linearized can you mix zoloft with pot mlspdna conjugate top row a and b red channel, middle row can you mix zoloft with pot c and d green channel, bottom row e and f corresponding overlaid images figure shows confocal fluorescence micrographs of cells can you mix zoloft with pot incubated with mlspdna conjugates, which were vectorized with vesicles made from the cyclohexyl derivative of dequalinium cdqasomes for the cell exposures can you mix zoloft with pot imaged in the left column panels �, � and e can you mix zoloft with pot the nonrestricted, ie circular form of pdna was used, while for can you mix zoloft with pot the experiments pictured in the right column panels b, d can you mix zoloft with pot and f, the plasmid dna was linearized before dqaplex formation can you mix zoloft with pot the characteristic red mitochondrial staining pattern panels a and b shows the functional viability of the imaged cells and the intracellular green motrin ib what is it fluorescence panels � and d demonstrates efficient cell internalization of the fluorescein labeled dna the green and red fluorescence channels were then overlaid to produce the composite image seen in panels e and f, where the regions of true colocalization of red and green fluorescence were pseudocolored in white for better visualization strikingly, in the overlaid images, there is hardly any can you mix zoloft with pot green fluorescence detectable nearly all areas of green fluorescence in panels � and d appeared as white areas in panels e can you mix zoloft with pot and f, strongly suggesting that almost the entire dna has can you mix zoloft with pot been delivered not only towards mitochondria, but also into the organelle however, whether all or at least a portion of the can you mix zoloft with pot pdna has actually entered the mitochondrial matrix, ie has crossed both mitochondrial membranes, and therefore would potentially be accessible to the mitochondrial transcription machinery, remains yet to be determined dqasomes as carriers of proapoptotic drugs dysregulation of the apoptotic machinery is generally accepted as an almost universal component of the transformation can you mix zoloft with pot process of normal cells into cancer cells and a large body of experimental data demonstrates that mitochondria play a key role can you mix zoloft with pot in the complex apoptotic mechanism consequently, any therapeutic strategy aimed can you mix zoloft with pot at specifically triggering apoptosis in cancer cells is believed to can you mix zoloft with pot have potential therapeutic effect, several clinically approved drugs such as vp can you mix zoloft with pot etoposide, arsenite and vinorelbine, as well as an increasing number of experimental anticancer drugs reviewed by constantini et al, such can you mix zoloft with pot as betulinic acid, lonidamine, ceramide and cd have been found to act directly on mitochondria, resulting in triggering apoptosis in order can you mix zoloft with pot to maximize the therapeutic potential of such anticancer drugs, which can you mix zoloft with pot are known to act at or inside mitochondria, the use of dqasomes as a mitochondriaspecific drug delivery system has been proposed hypothetically, dqasomebased anticancer chemotherapy entails features which would make it putatively superior to conventional chemotherapeutic approaches on the cellular, as well as the subcellular level firstly, the delivery of drugs known to act directly on mitochondria may trigger apoptosis in circumstances in which conventional drugs fail to act, because endogenous, upstream of mitochondria apoptosis induction pathways are disrupted secondly, transporting the cytotoxic can you mix zoloft with pot drug to its intracellular target could overcome multidrug resistance by hiding the drug inside the delivery system until it becomes selectively can you mix zoloft with pot released at the particular intracellular site of action, ie mitochondria thirdly, many carcinoma cells, including human breast adenocarcinoma derived cells, have an elevated plasma membrane potential relative to their normal parent cell lines in addition to the higher mitochondrial membrane potential, can you mix zoloft with pot they could provide the basis for a doubletargeting effect of can you mix zoloft with pot dqasomes, ie on the cellular level normal cells vs carcinoma cells, and on the subcellular level mitochondria versus nucleus first data involving the encapsulation of anticancer drugs into dqasomes have been can you mix zoloft with pot published most recently in this study, paclitaxel was chosen as can you mix zoloft with pot a model compound paclitaxel is known as a potent antitubulin agent used in the treatment of malignancies its therapeutic potential, however, is limited due to a very narrow span between the maximal tolerated dose and intolerable toxic levels in addition, its poor aqueous solubility requires the formulation of emulsions containing cremophor el�, can you mix zoloft with pot an oil of considerable toxicity by itself recently, it has been demonstrated that clinically relevant concentrations of paclitaxel target mitochondria directly and trigger apoptosis by inducing cytochrome � release in a can you mix zoloft with pot permeability transition pore ptpdependent manner this mechanism of action is can you mix zoloft with pot known from the other proapoptotic, directly on mitochondria acting agents a hour delay between the treatment with paclitaxel or with other can you mix zoloft with pot ptp inducers, and the release of cytochrome � in cellfree systems, compared with intact cells, has been explained by the existence can you mix zoloft with pot of several drug targets inside the cell, making only a subset of the drug available for mitochondria consequently, paclitaxel was considered a prime candidate to benefit from a mitochondriaspecific drug delivery system such as dqasomes it was demonstrated that paclitaxel can be incorporated into dqasomes at a stoichiometric molar ratio of paclitaxel to dequalinium considering the known spherical character of dqasomes, the results of an electron microscopic em analysis of dequasomal incorporated can you mix zoloft with pot paclitaxel, however, seem rather surprising the transmission em image fig , left panel and the cryoem image fig of an identical can you mix zoloft with pot sample show a remarkable conformity worm or rodlike structures approximately nm can you mix zoloft with pot in length, the size of which could also be confirmed by the size distribution analysis shown in fig , right panel the molecular structureof this wormlike complex remains to be determined nevertheless can you mix zoloft with pot fig left panel transmission electron microscopic image uranyl acetate staining can you mix zoloft with pot of dqasomal incorporated paclitaxel mol taxolmol dequalinium right panel size can you mix zoloft with pot distribution analysis of identical preparation shown in left panel the formation of wormlike micelles as described for selfassembling amphiphilic block copolymers appears possible � s � i in a preliminary study, can you mix zoloft with pot paclitaxelloaded dqasomes were tested for their ability to inhibit the growth can you mix zoloft with pot of human colon cancer cells in nude mice for controls can you mix zoloft with pot with free paclitaxel, the drug was suspended in dmso at mm, stored at �c and immediately diluted in warm medium before use in all controls, the respective dose of free paclitaxel can you mix zoloft with pot and empty dqasomes was adjusted according to the dose of paclitaxel and dequalinium given in the paclitaxelloaded dqasome sample due to the lack of any inhibitory effect on tumor growth, the can you mix zoloft with pot dose was tripled after weeks figure shows that at concentrations can you mix zoloft with pot where free paclitaxel and r hepes buffer v free paclitaxel empty dqasomes ?